Chemical Composition of Exosporium from Spores of Bacillus cereus

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Chemical composition of exosporium from spores of Bacillus cereus.

Homogeneous fragments of exosporium were extricated in centigram amounts from dormant spores of Bacillus cereus and analyzed for intrinsic constituents. The membrane proved to be chemically complex but not unique, consisting mainly of protein (52%), amino and neutral polysaccharides (20%), lipids (18%), and ash (4%). Seventeen common amino acids were identified by chromatography, and were prese...

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Ultrastructure of the Exosporium Enveloping Spores of Bacillus Cereus.

Gerhardt, Philipp (The University of Michigan, Ann Arbor), and Edgar Ribi. Ultrastructure of the exosporium enveloping spores of Bacillus cereus. J. Bacteriol. 88:1774-1789. 1964.-Structural details in the outer envelope of spores, such as those of Bacillus cereus and B. anthracis, were studied by electron microscopy and by X-ray diffraction analysis. Procedures were developed for isolating hom...

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In some Bacillus species, manganese levels influence the resistance properties of spores. To determine if this was true for Bacillus cereus, bacteria were sporulated with different MnCl2 concentrations resulting in spores with 30-fold differences in core Mn 2+ levels. Spores with different Mn 2+ levels displayed no differences in resistance to dry heat, UV radiation, γ-radiation, or hydrogen pe...

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The biphasic nature of germination curves of individual Bacillus cereus T spores was further characterized by assessing the effects of temperature, concentration of germinants, and some inorganic cations on microgermination. Temperature was shown to affect both phases of microgermination as well as the microlag period, whereas the concentration of l-alanine and supplementation with adenosine ex...

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A study was made of the activation of Bacillus cereus strain T spores by using the oxidizing agent sodium perborate. The degree of activation was measured with constant germination conditions by using L-alanine, inosine, adenosine, and L-alanine plus adenosine as germination stimulants. The germinal response following the various treatments was compared with the responses obtained with heat act...

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ژورنال

عنوان ژورنال: Journal of Bacteriology

سال: 1970

ISSN: 0021-9193,1098-5530

DOI: 10.1128/jb.101.1.196-201.1970